Technical Data Sheet: Stabilized Pinealon Tripeptide (Lyophilized)

1. Executive Summary for Laboratory Managers

This document provides essential information regarding the handling, storage, and reconstitution of the Stabilized Pinealon Tripeptide (Glu-Asp-Arg) to ensure maximum product stability and experimental reliability. This tripeptide offers high purity and robust structural stability, making it an ideal choice for diverse, long-term laboratory research applications.

2. Product Overview

2.1 Product Description

Stabilized Pinealon Tripeptide is a synthetic, short-chain peptide with the sequence Glu-Asp-Arg. It is supplied in a lyophilized (freeze-dried) state to maximize shelf life and stability. Its low molecular weight and inherent structural resistance make it a highly reliable reagent for cell culture, in vivo studies, and biochemical assays.

2.2 Product Highlights

• Chemical Structure: A highly stable short-chain peptide (Glu-Asp-Arg) ideal for diverse experimental setups.
• Form: Lyophilized powder for extended storage life.
• Intended Use: For Laboratory Research Only. Not for human or animal therapeutic use.

3. Specifications

The table below outlines the critical quality specifications of the Stabilized Pinealon Tripeptide batch.

Parameter

Specification

Method of Analysis

Sequence

Glu-Asp-Arg

Mass Spectrometry

Purity

>98%

HPLC

Appearance

White crystalline powder

Visual Inspection

Solubility

Soluble in water and saline.

Gravimetric/Visual

Molecular Weight

[Insert specific molecular weight]

Calculation

4. Storage Best Practices and Stability

Proper storage is crucial for maintaining the biological activity and purity of the peptide. Adherence to these guidelines is mandatory for reliable experimental outcomes.

4.1 Lyophilized (Powder) Storage

The lyophilized form offers maximum stability.

Storage Condition

Recommended Duration

Key Notes

Room Temperature (RT)

3–4 months

Store away from direct light and moisture.

Long-Term Storage

Store at -80°C

Maximum recommended shelf life: Date years.

Refrigerator (2–8°C)

Up to 1 year

Suitable for intermediate storage if -80°C is unavailable.

Stability Note: The tripeptide structure is naturally resistant to rapid enzymatic degradation compared to longer peptide chains, contributing to its robust shelf stability.

4.2 Reconstituted (Liquid) Storage

Reconstitution requires careful selection of the solvent and strict temperature control.

Storage Condition

Recommended Duration

Key Notes

Refrigerator (2–8°C)

Use within 30 days

Minimize freeze-thaw cycles. Aliquot if necessary.

Long-Term Liquid Storage

Not Recommended

Repeated freezing and thawing can compromise peptide integrity.

Important Solvents: Use bacteriostatic water (WFI with 0.9% benzyl alcohol) for reconstitution to inhibit microbial growth, especially if the reconstituted solution will be stored for up to 30 days.

5. Reconstitution Protocol

This protocol is designed to minimize degradation and ensure accurate concentration.

5.1 Required Materials

• Stabilized Pinealon Tripeptide vial
• Sterile, appropriate solvent (e.g., Bacteriostatic Water or Sterile Saline)
• Micropipette and sterile tips
• Vortex mixer (optional)

5.2 Step-by-Step Procedure

1. Warm-up: Allow the lyophilized vial to equilibrate to room temperature for at least 30 minutes before opening. This prevents condensation from forming inside the vial, which can reduce stability.
2. Determine Target Concentration: Calculate the exact volume of solvent needed to reach your desired stock concentration (e.g., 10 mg/mL).
3. Add Solvent: Slowly add the predetermined volume of solvent to the vial, directing the flow down the side of the glass to avoid splashing the powder.
4. Dissolve: Gently swirl the vial or use a low-setting vortex mixer until the peptide is completely dissolved. Do not shake vigorously, as this can cause foaming and potential denaturation.
5. Aliquoting (Recommended): For extended use, immediately divide the stock solution into single-use or small-volume aliquots. This minimizes the impact of freeze-thaw cycles if storing the solution below 0°C.
6. Labeling: Clearly label each aliquot with the peptide name, concentration, date of reconstitution (Date), and the name of the lab technician (Person).

6. Safety and Handling

6.1 General Handling Precautions

• Wear appropriate Personal Protective Equipment (PPE), including lab coats, gloves, and safety goggles.
• Handle the powder under a chemical fume hood to prevent inhalation of fine particles.
• Consult the relevant Safety Data Sheet (SDS) (File) before handling.

6.2 Emergency Procedures

Incident

Action Required

Eye Contact

Flush eyes with water for 15 minutes.

Skin Contact

Wash affected area with soap and water.

Ingestion

Do not induce vomiting. Seek immediate medical attention.

Spill

Wear appropriate PPE. Collect spilled material and dispose of in accordance with laboratory waste protocols.

7. Quality Assurance and Documentation

The stability and purity of this product are continuously monitored.

7.1 Batch Information

Parameter

Value

Batch Number

[Insert Batch Number]

Date of Manufacture

Date

Quality Control Manager

Person

7.2 Related Documentation

Access the following documents for more comprehensive information:

• Certificate of Analysis (CoA): File
• SDS: File
• Pinealon Tripeptide Assay Protocol: File

8. Troubleshooting and Support

Issue

Possible Cause

Recommended Action

Peptide does not dissolve fully.

Incorrect solvent or insufficient mixing.

Ensure correct solvent (water/saline) and gently increase mixing time/intensity. Allow more time for full dissolution.

Loss of activity post-reconstitution.

Repeated freeze-thaw cycles or storage above 8°C.

Aliquot stock solution and store at 2–8°C for a maximum of 30 days. Discard solutions stored improperly.

Contamination observed.

Non-sterile technique or use of non-bacteriostatic water.

Reconstitute using strictly sterile technique and use bacteriostatic water for stock solutions.

For technical support or to schedule a consultation regarding your experimental setup, please contact the support team at [Insert Support Email] or attend the scheduled technical briefing (Calendar event).

9. Regulatory Compliance and Disclaimer

9.1 Regulatory Status

This product is sold strictly as a research reagent. It has not been evaluated by any regulatory body for use in humans or as a diagnostic or therapeutic agent.

9.2 Liability Statement

The user assumes all responsibility for the proper handling and storage of this product. The supplier (Person) is not liable for any damages or losses resulting from misuse or non-adherence to the guidelines specified herein.

10. Appendix: Resources and Contact Information

10.1 Key Contacts

Role

Name

Contact

Lab Manager

Person

[Insert Lab Manager Contact]

Procurement Officer

Person

[Insert Procurement Contact]

Technical Support

Person

[Insert Tech Support Contact]

10.2 Training and Workshops

Mandatory refresher training on peptide handling and reconstitution techniques is held annually at the main campus laboratory (Place). The next session is scheduled for Date (Calendar event).